If every 6th section is used, then around 13 sections are used. Suppose that the tissue is sectioned into 80 sections. A possible starting point for a pilot study might be 10 to 15 sections. The sections are sampled using the fractionator principle. The Cavalieri estimator is prone to overprojection if the set of slices are thick. The following is an example of a pilot study but the number of sections and number of points will depend on your material.īegin by preparing a set of sections. How many sections and how many points per section are needed? There are several ways to think about this (see precision), and come up with a starting point for a pilot study. The precision of sampling should also be considered. If you follow the rules you will arrive at an unbiased estimate of volume. The Cavalieri estimator is used to estimate volume.
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